FlyCut® KpnI is expressed and purified from E.coli that carries the recombinant KpnI gene. The molecular weight is 25.9 kDa, with the recognition site at GGTAC^C. The reaction is conducted for 5-15 minutes at 37℃, and heat-inactivated at 80℃ for 20 minutes. This enzyme is not sensitive to dam, dcm or mammalian CpG methylation.

Recognition Site
5’...GGTAC↓C...3’
3’...C↑CATGG...5’

Applications
Genomic DNA, plasmid DNA, PCR product

Storage
at -20°C  for two years

Shipping
Dry ice (-70 ℃)