The P2 Primary Cell 4D-NucleofectorTM X Kit S is one of our five 4D-NucleofectorTM Kits suited for transfecting primary cells in the 20 µL 16-well NucleocuvetteTM Strip format when working with the 4D-NucleofectorTM X Unit.
This kit has been determined to be optimal for transfecting following cell types:
The respective cell type-specific Optimized Protocols or recommendations are available under "Instructions" or in our knowledge database. In these Optimized Protocols the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.
2 x 0.675 mL P2 Primary Cell NucleofectorTM Solution2 x 0.15 mL Supplement 150 µg pmaxGFPTM Vector (1 μg/μl in 10 mM Tris pH 8.0)2 x 16-well NucleocuvetteTM Strips (20 μl)