Sample type

Plasma, serum, urine.

Starting material

Up to 100 ul of biological sample can be loaded per well.

Unfractionated plasma sample can be directly used for capture.

Concentrated urine samples are recommended prior capture according to our suggested protocol.

Assay type

Sandwich enzyme-linked immunoassay.

Sensitivity

The detection limit of an assay is lower than 0.35 ug of overall exosome which is an equivalent of less than 50 pg of targeted exosomes protein.

Product overview

ExoTEST Ready to Use Kit is the ideal platform for capturing and the quantifying exosomes from biological samples such as plasma, serum, urine and cell culture supernatant. 

ExoTEST Ready to Use Kit features pre-coated ELISA plate with primary antibodies detection directed against exosome-specific markers, namely α-CD9. Secondary antibodies and substrate reagents are included in the kit.

Exosome standards for assay calibration are also included in the kit. Exosome standards are purified by ultracentrifugation and microfiltration, quantified for overall protein content and exosome number and then lyophilized for long-term storage.

ExoTEST ready to use kits are reliable, easy to use, works on a variety of commercially available detection technologies.

Primary antibody

α-CD9 murine antibody.

Exosome standard

Lyophilized exosomes from COLO-1 Cell culture supernatant (see details).

Assay protocol

Kit contains all the reagents necessary for exosome capture and protein targeted detection.

Assay calibration: kit contains exosome standards for assay calibration. All exosome standards are lyophilized and quantified for overall protein content and exosomes number.

This standards enable both absolute and relative quantification exosomes in analyzed samples.

Shipment and storage

All the reagents are shipped at 4C and storage conditions as recommended in the product insert.

Expiration date

12 months.