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Enzyme Activity:
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Isoamylase
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EC Number:
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3.2.1.68
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CAZy Family:
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GH13
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CAS Number:
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9067-73-6
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Synonyms:
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isoamylase; glycogen 6-alpha-D-glucanohydrolase
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Source:
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Pseudomonas sp.
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Molecular Weight:
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71,500
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Concentration:
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Supplied at ~ 200 U/mL
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Expression:
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Purified from Pseudomonas sp.
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Specificity:
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Hydrolysis of (1,6)-α-D-glucosidic branch linkages in glycogen, amylopectin and their β-limit dextrins.
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Specific Activity:
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~ 180 U/mg (40oC, pH 4.0 on oyster glycogen) (equivalent to 16 MU Sigma Units/mg)
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Unit Definition:
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One unit of isoamylase activity is the amount of enzyme required to release one µmole of D-glucose reducing sugar equivalent in the presence of oyster glycogen per min at pH 4.0 and 40oC.
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Temperature Optima:
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50oC
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pH Optima:
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4
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Application examples:
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Applications in carbohydrate research and in the food and feeds, and cereals industry.
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Method recognition:
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AOAC Method 2000.11 and GB Standard 5009.245-2016
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