The MycoAlertTM Mycoplasma Detection Kit (100 Tests) is a selective biochemical test that exploits the activity of mycoplasmal enzymes which are found in all six of the main mycoplasma cell culture contaminants and the vast majority of 180 mycoplasma species, but are not present in eukaryotic cells. Viable mycoplasma in a test sample (cell supernatant) are lysed and the enzymes react with the MycoAlertTM Substrate, catalyzing the conversion of ADP to ATP. The ATP is then transferred into a light signal via the luciferase enzyme in the MycoAlertTMReagent. By measuring the level of ATP in a sample both before (read A) and after the addition of the MycoAlertTM Substrate (read B), a ratio can be obtained which is indicative of the presence or absence of mycoplasma.
1 x 10 mL MycoAlertTM Reagent (lyophilized) 1 x 10 mL MycoAlertTM Substrate (lyophilized) 1 x 20 mL MycoAlertTM Assay Buffer
