General description

β-Glucuronidase (GUSB) is a lysosomal enzyme, found in animals, plants and bacteria. This exoglycosidase shows high activity in spleen, liver and kidney. Bovine liver β-glucuronidase (BLG) is homotetramer with a molecular weight of 290 kDa.

Application

β-Glucuronidase from bovine liver has been used in β-glucuronidase inhibition assay to study the β-glucuronidase inhibitory effect of extracts and isolated actives from Phyllanthus amarus. It has also been used to perform deglucuronidation.

β-葡萄糖醛酸酶在GUS测定中可用作报告基因以监测基因的表达。

包装

100000, 500000, 1000000, 5000000 units in poly bottle

Biochem/physiol Actions

β-Glucuronidase (GUSB) deficiency can result in mucopolysaccharidosis type VII (MPSVII), which leads to store lysosome in the brain. Integration of β-glucuronides of steroid hormones into tissues can be done with the help of GUSB.

β-葡萄糖醛酸酶可催化复合碳水化合物的分解。在人体中，它可将结合胆红素转化为非结合的形式，使胆红素利于重吸收。

Unit Definition

在37℃，pH 5.0（30分钟检测）条件下，一个Sigma或改良的Fishman单位可每小时从酚酞葡糖苷酸中释放1.0 μg酚酞。