General description

Chitinase is an extracellular complex of enzymes that degrade chitin. Chitin is a cell wall component of Fungi and exoskeketal essentials of different organisms which reshape their own chitin or digest/dissolve the chitin of other organisms (insects, fungi, yeast, and algae, and in the internal structures of other vertebrates) . Chitinases have been detected in many microorganisms and in plants. In fungi, chitinases assist in morphogenesis, to break down the inherent chitin content of fungal cell walls. Plant chitinases help in resistance to fungal attack and counteracting fungal growth, by targeting those same fungal cell walls. In bacteria, bacterial chitinases assist in utilizing chitin as a carbon source and as an energy source.Streptomyces griseus produces multiple chitinases of different molecular masses after growth induction with chitin as the carbon source.
The enzymatic hydrolysis of chitin to N-acetyl-D-glucosamine involves two consecutive enzyme reactions:
• The first reaction, chitodextrinase-chitinase, is a poly(β-(1→4)-[2-acetamido-2-deoxy-D-glucoside])- glycanohydrolase, which removes chitobiose units from chitin.
• The second activity is N-acetyl-glucosaminidasechitobiase, which cleaves the disaccharide to its monomer subunits, N-acetyl-D-glucosamine.

Application

Agriculture fields: control pathogens.
Human health care: Asthma.
Pharma: preparation of chitooligosaccharides and N-acetyl D glucosamine,
Preparation of single-cell protein
Isolation of protoplasts from fungi and yeast
Control of pathogenic fungi
Treatment of chitinous waste, mosquito control and morphogenesis

包装

5, 25, 50 units in glass bottle

Biochem/physiol Actions

几丁质酶是一种细胞外酶复合物，可降解几丁质，分子量约为30 kDa。几丁质经过2个酶促反应，被降解为N-乙酰基-D-葡糖胺。首先，壳二糖酶-几丁质酶去除壳多糖中的壳二糖单元。在第二个反应中，N-乙酰基-氨基葡糖苷酶-壳二糖酶将二糖切割成其单体亚基（由N-乙酰基-D-葡糖胺组成）。最佳反应温度为37°C。

Features and Benefits

Chitinase is an extracellular complex of enzymes that degrade chitin. It is a lytic enzyme suitable for fungal cell walls lysis.

Unit Definition

一个酶活性单位是指在2小时测定中，在pH6.0、25℃条件下每小时分解几丁质释放1.0mg N-乙酰基-D-葡糖胺所需的酶量。
一个新1小时单位=约50个48小时单位。