Antibody TypeMonoclonalHost SpeciesArmenian HamsterImmunogenTrinitrophenol + KLHFormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.PreparationThe immunoglobulin was purified by affinity chromatography.Concentration0.5 mg/mlStorage & HandlingThe antibody solution should be stored undiluted between 2°C and 8°C.Application
FC, ICFC - Quality tested
IP - Validated
ICC, IHC, WB, FA - Reported in the literature
Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control, and the purity is greater than 95% by SDS-PAGE. Use at concentrations comparable to those of the specific antibody of interest. For immunoprecipitation, the suggested use of this reagent is 2.5 µg/test.
Application Notes
Additional reported applications (for the relevant formats) include: Intracellular Flow Cytometry (ICFC), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB), Functional Assay (FA)
For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 400940) with a lower endotoxin limit (Endotoxin < 0.01 EU/µg).
Application References
(PubMed link indicates BioLegend citation)
- Lesley R, et al. 2006. P. Natl. Acad. Sci. USA 103:10717.
- Yu R, et al. 2006. Obesity 14:1353.
- Yang JH, et al.2005. Rheumatology(Oxford). 44:1245. PubMed
- Mina-Osorio P, et al. 2008. J. Leukocyte Biol. 84:448. PubMed
- Shen H, et al. 2009. J. Am Soc Nephrol. 20:1032. PubMed
Product Citations
- Yang J, et al. 2005. Rheumatology. 44:1245. PubMed
- Goldstein H 2009. J Am Soc Nephrol. 1.55. PubMed
- Bechelli J, et al. 2019. Infect Immun. 87:. PubMed
- Mantri CK, et al. 2019. J Clin Invest. 129:1094. PubMed
- Olcina MM et al. 2018. Cell reports. 25(13):3721-3732 . PubMed
- Kalamakis G, et al. 2019. Cell. 176:1407. PubMed
- Nie S, et al. 2019. Cancer Manag Res. 11:10257. PubMed
